Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
نویسندگان
چکیده مقاله:
This research has focused on isolation and characterization of a strain of Bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at pHs ranging from 8 to 11 and temperatures of 20 to 50ºC. Also the impact of different carbon and nitrogen sources were investigated. The yield and fold of enzyme purification was 24% and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline protease produced by Bacillus sp. 2 - 5 showed the most caseinolytic activity (without any gelatinolytic activity) at pH>10
منابع مشابه
production and purification of a protease from an alkalophilic bacillus sp. 2-5 strain isolated from soil
this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight o...
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عنوان ژورنال
دوره 5 شماره 2
صفحات 110- 113
تاریخ انتشار 2007-04-01
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